Designed to transfect a broad range of eukaryotic cells, including insect cells, many cell lines not transfected well by other reagents, and hardtotransfect cell lines, such as ht1080, k562, and hepg2. Xtremegene hp dna transfection reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hardtotransfect. Both lipofectamine and a previous version of xtremegene hp, fugene were. Xtremegene hp dna transfection reagent protocol sigma. Complex formation at lower volumes can significantly decrease transfection efficiency. Of the four tested reagents, xtremegene hp delivered the best transfection results, which were comparable to those of other studies that used the reagent or its predecessor fugene on z3 embryonic fibroblasts 18 or epc carp cells. Xtremegene hp dna transfection reagent is a multicomponent reagent that forms a complex with dna, then transports the complex into animal or insect cells. Xtremegene 9 dna transfection reagent sigmaaldrich.
Xtremegene hp dna transfection reagent highperformance polymer reagent for transfecting many cell lines. Polymerbased reagent for delivering dna, sirna, mirna and crisprrnp into many cells 1. A method to evaluate the efficiency of transfection. Each lot of xtremegene hp dna transfection reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. X tremegene hp dna transfection reagent, supplied by roche, used in various techniques. The reagent remains fully functional even after repeated opening of the vial at least. Gfp expression was visualized 48 hours after transfection. Xtremegene hp dna transfection reagent protocol sigmaaldrich. Xtremegene hp dna transfection reagent highperformance. Xtremegene transfection reagents general recommendations.
Allow xtremegene 9 dna transfection reagent, dna, and diluent. Xtremegene hp dna transfection reagent is a high performance transfection reagent, free of animalderived components. Dna deliverytransient and stable transfection used to study gene function and regulation, mutational analysis and biochemical characterization of gene products, effects of gene expression on the health and life cycle of cells, as well as for large scale production of proteins for purification and downstream applications. During quality testing, cells are transfected with a reporter gene vector dna using xtremegene hp dna transfection reagent ratio 3. Plate freshly passaged cells at optimal concentration. Xtremegene hp dna transfection reagent outperforms reagent ltx p. X tremegene hp dna transfection reagent roche bioz ratings. Xtremegene transfection reagents from roche have been used for a wide range of applications and a vast array of cell lines, primary cells and stem cells.
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